A mass balance and kinetic investigation of anaerobic dechlorination of pentachlorophenol (PCP) was undertaken using
an enriched microbial consortium in a laboratory scale continuous flow column, as a model microbial permeable reactive
barrier. The chlorine balance showed that 50 μM PCP was largely dechlorinated to phenol with the formation of a small
quantity of 3-chlorophenol as an intermediate metabolite (hydraulic retention time 7.6 days), and the chlorine removal
efficiency reached 36 μM d–1. When the initial PCP concentration was increased to 100 μM the chlorine removal efficiency
increased 1.5 times. However, the dechlorination activity disappeared after 7.4 pore volumes (58 days), demonstrating the
susceptibility of the dechlorination culture to high concentrations of PCP. Lactate released hydrogen as an electron donor
during PCP dechlorination, with acetate, propionate, CO2 and CH4 as byproducts. The carbon balance showed that some of the
organic carbon source (PCP, lactate) in the influent was converted to gas and utilized for biomass growth in addition to
organic metabolites. The kinetic study was conducted in a batch culture and yielded 1.99 mg l–1 biomass growth per unit of
chlorine consumption (μM). The Monod equation was well fitted to the specific growth rate of 1.38 d–1 and a half saturation
constant of 0.29 μM. The organic chlorine removal rate in the batch culture was consistent with the results in the flow column,
indicating the feasibility of and potential for in situ estimation and prediction through batch culture studies.
รบกวนช่วยแปลบทความเกี่ยวกับวิทยาศาสตร์หน่อยค้า เอาสรุปรวมๆเลยค้าไม่ต้องแปะทุกประโยคแต่อ่านแล้วเข้าใจอะค้า ขอบคุณค้า
an enriched microbial consortium in a laboratory scale continuous flow column, as a model microbial permeable reactive
barrier. The chlorine balance showed that 50 μM PCP was largely dechlorinated to phenol with the formation of a small
quantity of 3-chlorophenol as an intermediate metabolite (hydraulic retention time 7.6 days), and the chlorine removal
efficiency reached 36 μM d–1. When the initial PCP concentration was increased to 100 μM the chlorine removal efficiency
increased 1.5 times. However, the dechlorination activity disappeared after 7.4 pore volumes (58 days), demonstrating the
susceptibility of the dechlorination culture to high concentrations of PCP. Lactate released hydrogen as an electron donor
during PCP dechlorination, with acetate, propionate, CO2 and CH4 as byproducts. The carbon balance showed that some of the
organic carbon source (PCP, lactate) in the influent was converted to gas and utilized for biomass growth in addition to
organic metabolites. The kinetic study was conducted in a batch culture and yielded 1.99 mg l–1 biomass growth per unit of
chlorine consumption (μM). The Monod equation was well fitted to the specific growth rate of 1.38 d–1 and a half saturation
constant of 0.29 μM. The organic chlorine removal rate in the batch culture was consistent with the results in the flow column,
indicating the feasibility of and potential for in situ estimation and prediction through batch culture studies.