ช่วยแปลวิธีการทดลองตอนนี้ให้หน่อยค่ะ ไม่ค่อยเข้าใจวิธีการเลยค่ะ
Larvicidal tests
Standard methods for testing the larvicidal action of the prepared tablets were slightly modified from the WHO (1996). A stock solution was prepared by dissolving tablets containing 5% and 10% R. nasutus extract concentration in distilled
water in a volumetric flask and stored in a refrigerator at 15ºC. After 20 healthy, late 3rd-early 4th instar larvae were introduced into each experiment cup [sterilized drinking plastic cup (150 ml capacity)], which contained 100 ml of dechlorinated water, an appropriate volume of stocksolution was added to obtain the desired concentration.
Experiments were carried out in a series of five concentrations in 5 replicates with the final total number of 100 larvae for each concentration. Each replicate set contained one control, which was 100 ml of dechlorinated water alone, and the other control was 100 ml of water containing a maximum volume of lactose solvent in the test medium. Since very few larvae succumbed within 24 hours of exposure to the tested solutions, the mortality was recorded at 48 hours of exposure, during which no food was offered to the larvae. The mortalities of mosquito larvae were recorded according to the following criteria: moribund larvae, which were incapable of rising to the surface or did not show the characteristic diving reaction when the water was disturbed, had discoloration, an unnatural position or rigor. The LC50 and LC90 values were determined by the Probit analysis program (Finney, 1971). The control mortality rate was accounted for by Abbott’s formula (Abbott,
1925).
****ช่วยหนูด้วยนะคะ ขาดแค่ตอนนี้ตอนเดียวก็จะสมบูรณ์แล้วค่ะ
ใคนเก่งภาษาอังกฤษช่วยหนูหน่อยค่ะ แปลเปเปอร์
Larvicidal tests
Standard methods for testing the larvicidal action of the prepared tablets were slightly modified from the WHO (1996). A stock solution was prepared by dissolving tablets containing 5% and 10% R. nasutus extract concentration in distilled
water in a volumetric flask and stored in a refrigerator at 15ºC. After 20 healthy, late 3rd-early 4th instar larvae were introduced into each experiment cup [sterilized drinking plastic cup (150 ml capacity)], which contained 100 ml of dechlorinated water, an appropriate volume of stocksolution was added to obtain the desired concentration.
Experiments were carried out in a series of five concentrations in 5 replicates with the final total number of 100 larvae for each concentration. Each replicate set contained one control, which was 100 ml of dechlorinated water alone, and the other control was 100 ml of water containing a maximum volume of lactose solvent in the test medium. Since very few larvae succumbed within 24 hours of exposure to the tested solutions, the mortality was recorded at 48 hours of exposure, during which no food was offered to the larvae. The mortalities of mosquito larvae were recorded according to the following criteria: moribund larvae, which were incapable of rising to the surface or did not show the characteristic diving reaction when the water was disturbed, had discoloration, an unnatural position or rigor. The LC50 and LC90 values were determined by the Probit analysis program (Finney, 1971). The control mortality rate was accounted for by Abbott’s formula (Abbott,
1925).
****ช่วยหนูด้วยนะคะ ขาดแค่ตอนนี้ตอนเดียวก็จะสมบูรณ์แล้วค่ะ